Cryopreservation of common carp Cyprinus carpio and tench Tinca tinca sperm for gene resources conservation
Linhart, O.; Rodina, M. (2000). Cryopreservation of common carp Cyprinus carpio and tench Tinca tinca sperm for gene resources conservation, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 402-404
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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| Abstract |
In this study, cryopreservation methods were elaborated for ex situ conservation of Bohemian common carp and tench (7 strains of common carp and 9 strains of tench). Sperm of tench is usually contaminated with urine, therefore the sperm was collected directly into modified extenders of Kurokura. Common carp sperm was diluted 1:5 in a Kurokura medium and equilibrated at 4 degree C. Diluted sperm of both species was transferred to 2 ml cryotubes and 10% of DMSO was added, than the cryotubes were directly transferred to pre-programmed PLANER Kryo 10 series III and cooled from +4 degree C to -9 degree C at a rate of 4 degree C. min super(-1), then from 9 degree C to -80 degree C at a rate of 11 degree C .min super(-1), hold for 6 min at -80 degree C and finally transferred into liquid N sub(2). The spermatozoa were thawed in a water bath 35 degree C for 110 s and checked for fertilization rate, hatching rate and larval malformations. Sperm motility was evaluated for the percentage and velocity of motile video frames. ANOVA showed significant influence of sperm (frozen and fresh P<0.0001) on fertilization rate and hatching, but insignificant on larval malformations 0-6.8% and insignificant influence of different males on fertilization rate, hatching and larval malformation. Multiple range analysis (LSD) assessed difference between frozen sperm and fresh sperm on fertilization rate 54.73% and 82.6% (P<0.01), hatching rate 50.58% and 59.44% (P<0.01), respectively in common carp. In tench, ANOVA showed significant influence of sperm (frozen and fresh P<0.0001) on hatching and insignificant influence of different groups of males on hatching. Larval malformation was zero. Multiple range analysis (LSD) assessed difference between frozen sperm at solution A, B, C and fresh sperm on hatching rate 25.49, 33.13, 34.84% and 86.92% (P<0.05), respectively. |
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