Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus
Godfroy, O.; Peters, A.F.; Coelho, S.M.; Cock, J.M. (2015). Genome-wide comparison of ultraviolet and ethyl methanesulphonate mutagenesis methods for the brown alga Ectocarpus. Marine Genomics 24(1): 109-113. https://dx.doi.org/10.1016/j.margen.2015.03.007
In: Marine Genomics. Elsevier: Amsterdam. ISSN 1874-7787; e-ISSN 1876-7478, meer
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| Trefwoorden |
Exploitable Scientific Result
Marine Sciences
Maritime Industries > Blue Biotech
Scientific Community
Scientific Publication
Marien/Kust |
| Author keywords |
Brown algae; Ectocarpus; Ethyl methanesulphonate; Mutagenesis;Ultraviolet |
| Project | Top | Auteurs |
- Association of European marine biological laboratories, meer
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| Auteurs | | Top |
- Godfroy, O.
- Peters, A.F.
- Coelho, S.M.
- Cock, J.M.
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| Abstract |
Ectocarpus has emerged as a model organism for the brown algae and a broad range of genetic and genomic resources are being generated for this species. The aim of the work presented here was to evaluate two mutagenesis protocols based on ultraviolet irradiation and ethyl methanesulphonate treatment using genome resequencing to measure the number, type and distribution of mutations generated by the two methods. Ultraviolet irradiation generated a greater number of genetic lesions than ethyl methanesulphonate treatment, with more than 400 mutations being detected in the genome of the mutagenised individual. This study therefore confirms that the ultraviolet mutagenesis protocol is suitable for approaches that require a high density of mutations, such as saturation mutagenesis or Targeting Induced Local Lesions in Genomes (TILLING). |
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