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Long-term acclimation of Pseudokirchneriella subcapitata (Korshikov) Hindak to different copper concentrations: changes in tolerance and physiology
Bossuyt, B.T.A.; Janssen, C.R. (2004). Long-term acclimation of Pseudokirchneriella subcapitata (Korshikov) Hindak to different copper concentrations: changes in tolerance and physiology. Aquat. Toxicol. 68(1): 61-74. dx.doi.org/10.1016/j.aquatox.2004.02.005
In: Aquatic Toxicology. Elsevier Science: Tokyo; New York; London; Amsterdam. ISSN 0166-445X; e-ISSN 1879-1514, meer
Peer reviewed article  

Beschikbaar in  Auteurs 
    VLIZ: Open Repository 280289 [ OMA ]

Trefwoorden
    Adaptation
    Chemical elements > Metals > Transition elements > Heavy metals > Copper
    Emissions > Pollutants
    Environments > Aquatic environment
    Heavy metals
    Properties > Biological properties > Tolerance
    Toxicity
    Toxicology > Ecotoxicology
    Water pollution
    Algae [Algen]; Chlorophyta [WoRMS]; Pseudokirchneriella subcapitata (Korshikov) F.Hindák, 1990 [WoRMS]
    Zoet water
Author keywords
    Pseudokirchneriella subcapitata; copper; acclimation; optimal concentration

Auteurs  Top 
  • Bossuyt, B.T.A., meer
  • Janssen, C.R., meer

Abstract
    The effect of long-term copper acclimation of the freshwater green algae Pseudokirchneriella subcapitata to copper was investigated using different physiological and toxicological endpoints. The algae were exposed to seven-five of which are ecologically relevant for European surface waters-copper concentration ranging from 0.5 to 100 µg Cu l-1 during a 3-month period. A standard medium was used as culture and test medium with an addition of 2 mg DOC l-1(replacing EDTA). At certain intervals, experiments were performed to assess algal biomass, growth rate, chlorophyll and carotenoid content, pigment diversity, autotrophic index, intracellular and adsorbed copper, and the sensitivity of the algae to copper. Chronic copper tolerance (mean ± standard deviation) increased significantly from 88 ± 15 to 124 ± 25 µg Cu l-1 for P. subcapitata acclimated to 0.5 and 100 µg Cu l-1, respectively. Based on the algal biomass, the growth rate, the pigment diversity and the autotrophic index, an optimal concentration range was observed between 1 and 35 µg Cu l-1. Significant decreases in algal biomass, pigment diversity and autotrophic index were observed in algal cultures acclimated to 0.5 tag Cu l-1 and 100 tag Cu l-1. Chlorophyll a content (mean ± standard deviation) increased from 8.4 ± 3.1 to 28.6 ± 7.5 x 10-14 g per cell and carotenoid content (mean ± standard deviation) increased from 3.7 ± 0.8 to 7.1 ± 1.2 x 10-14 g per cell for algae exposed to 1 and 100 µg Cu l-1, respectively. Intracellular copper increased from 0.099 to 20.6 x 10-15 g Cu per cell and adsorbed copper increased from 0.026 to 1.8 x 10-1 g Cu per cell for algae acclimated for 12 weeks to 0.5 and 100 tag Cu l-1, respectively. This research demonstrates that the use of standard culture media, some of which may be deficient in copper, can result in sub-optimal performance of the organisms, which in turn may affect toxicity test results. Additionally, this work also established an optimal concentration range for copper for this algal species. This phenomenon should be taken in consideration when performing environmental risk assessments of essential elements.

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