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Development of bioanalytic tools for the evaluation of endocrine-modulating effects on the development and reproduction of zebrafish, Danio rerio
Fenske, M.; Maack, G.; Schäfers, C.; Schmitz, A.; Ensenbach, U.; Segner, H. (2000). Development of bioanalytic tools for the evaluation of endocrine-modulating effects on the development and reproduction of zebrafish, Danio rerio, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 389
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Fenske, M.
  • Maack, G.
  • Schäfers, C.
  • Schmitz, A.
  • Ensenbach, U.
  • Segner, H.
Abstract
    The establishment of histological and immunochemical methods to identify estrogen-sensitive stages in the development of zebrafish, Danio rerio, are described. Preliminary histological results indicate that the sensitive stage, when xenoestrogens may alter the normal sexual differentiation of the zebrafish occurs between hatching and day 66. For the measurement of the biomarker vitellogenin, zebrafish-homologous polyclonal antibodies were raised. These two antibodies were partially characterized by Western blot and used in non-competitive ELISA.
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