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The development of a quantitative RT-PCR assay to measure P450 aromatase mRNA expression in the fathead minnow, (Pimephales promelas) with a view to its application for studying endocrine disruption
Halm, S.; Sumpter, J.P.; Rand-Weaver, M.; Hutchinson, T.H.; Tyler, C.R. (2000). The development of a quantitative RT-PCR assay to measure P450 aromatase mRNA expression in the fathead minnow, (Pimephales promelas) with a view to its application for studying endocrine disruption, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 386
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Halm, S.
  • Sumpter, J.P.
  • Rand-Weaver, M.
  • Hutchinson, T.H.
  • Tyler, C.R.
Abstract
    A competitive, quantitative RT-PCR assay was set up to quantify expression of P450aromatase mRNA in the fathead minnow, with a view to examine effects of endocrine disrupting chemicals (EDCs) on this steroidogenic enzyme. Aromatase mRNA expression was detected in ovary, testis and brain of sexually maturing females and males. P450aromatase mRNA expression in the brain was 10-100 fold higher than in the gonads. Expression was dependent on the stage of sexual maturity. The development of a quantitative RT-PCR assay for P450aromatase mRNA and the provision of baseline data on its expression have provided us with the necessary tools to start to study gene expression of this enzyme in the fathead minnow.
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