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Gonadal formation and differentiation in a teleost, tilapia Oreochromis niloticus
Kobayashi, T.; Kajiura-Kobayashi, H.; Nagahama, Y. (2000). Gonadal formation and differentiation in a teleost, tilapia Oreochromis niloticus, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 259
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Kobayashi, T.
  • Kajiura-Kobayashi, H.
  • Nagahama, Y.
Abstract
    To clarify the characteristics of germ cells during differentiation and gametogenesis, we cloned tilapia vasa homologue (vas) cDNA. Using vas, we examined the expression of vas in germ cell stages from PGCs to gametogenesis. in situ hybridization for vas mRNA indicated that signals are present in migrated PGCs at 0 dah. PGCs continued to express vas mRNA after incorporation in the gonadal anlage. During oogenesis, vas transcripts were expressed strongly in stages from oogonia to diplotene oocytes. Thereafter, hybridization signals decreased in stages from vitellogenic oocytes to full grown oocytes.
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