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3ß-hydroxysteroid dehydrogenase gene: sites of expression in trout gonads and cAMP-dependent regulation
Young, G.; Todo, T.; Kusakabe, M.; Kobayashi, T.; Nagahama, Y. (2000). 3ß-hydroxysteroid dehydrogenase gene: sites of expression in trout gonads and cAMP-dependent regulation, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 202
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Young, G.
  • Todo, T.
  • Kusakabe, M.
  • Kobayashi, T.
  • Nagahama, Y.
Abstract
    Using cRNA in situ hybridization, weak to moderate expression of the 3 beta -hydroxysteroid dehydrogenase gene was shown to be confined to special thecal cells in thecal layers of rainbow trout vitellogenic follicles. Hybridization signals and positive thecal cells numbers increased in the post-vitellogenic period. Granulosa cells occasionally displayed weak hybridization signals. in vitro forskolin treatment enhanced hybridization signals in juvenile ovary and testis. Similar increases occurred in GTH- or forskolin-treated early vitellogenic follicles.
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