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Isolation and characterization of differentially expressed cDNAs in the ovulatory and post-ovulatory trout ovary
Bobe, J.; Goetz, F.W. (2000). Isolation and characterization of differentially expressed cDNAs in the ovulatory and post-ovulatory trout ovary, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 195
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Bobe, J.
  • Goetz, F.W.
Abstract
    Using differential display PCR (DDPCR) and subtractive PCR, 3 differentially expressed cDNAs were isolated from ovulatory and post-ovulatory ovaries of the rainbow trout (Oncorhynchus mykiss) and brook trout (Salvelinus fontinalis). One of the cDNAs, was originally isolated using DDPCR on phorbol ester stimulated brook trout ovaries. This cDNA presumably encodes for a 302 amino acid protein similar to several members of the tumor necrosis factor receptor (TNFR) family and was upregulated at the completion of ovulation in the trout ovary
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