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Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains
Lopez-Goni, I.; Garcia-Yoldi, D.; Marin, C.M.; de Miguel, M.J.; Munoz, P.M.; Blasco, J.M.; Jacques, I.; Grayon, M.; Cloeckaert, A.; Ferreira, A.C.; Cardoso, R.; Corrêa de Sa, M.I.; Walravens, K.; Albert, D.; Garin-Bastuji, B. (2008). Evaluation of a multiplex PCR assay (Bruce-ladder) for molecular typing of all Brucella species, including the vaccine strains. Journal of Clinical Microbiology 46(10): 3484-3487. https://dx.doi.org/10.1128/JCM.00837-08
In: Journal of Clinical Microbiology. AMER SOC MICROBIOLOGY: Washington. ISSN 0095-1137; e-ISSN 1098-660X, meer
Peer reviewed article  
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  • Lopez-Goni, I.
  • Garcia-Yoldi, D.
  • Marin, C.M.
  • de Miguel, M.J.
  • Munoz, P.M.
  • Blasco, J.M.
  • Jacques, I.
  • Grayon, M.
  • Cloeckaert, A.
  • Ferreira, A.C.
  • Cardoso, R.
  • Corrêa de Sa, M.I.
  • Walravens, K.
  • Albert, D.
  • Garin-Bastuji, B.
Abstract
    An evaluation of a multiplex PCR assay (Bruce-ladder) was performed in seven laboratories using 625 Brucella strains from different animal and geographical origins. This robust test can differentiate in a single step all of the classical Brucella species, including those found in marine mammals and the S19, RB51, and Rev.1 vaccine strains.
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