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Effects of recombinant human inhibin on GTH II secretion from dispersed pituitary cells of female rainbow trout during the reproductive cycle
Chyb, J.; Breton, B. (2000). Effects of recombinant human inhibin on GTH II secretion from dispersed pituitary cells of female rainbow trout during the reproductive cycle, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 472-474
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer

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  • Chyb, J.
  • Breton, B.

Abstract
    The influence of human recombinant inhibin on gonadotropin release was studied at different stages of female rainbow trout gametogenesis, using the culture of dispersed pituitary cells. Inhibin decreased the basal GtH I release in immature fish and during the vitellogenesis, having no effect at the beginning of vitellogenesis and before ovulation. After ovulation, it decreased the secretion of GtH I only in fish stripped of eggs, while it had no effect in unstripped females, suggesting the interference with factors contained in the ovarian fluid. The basal secretion of GtH II was stimulated by inhibin from the stage of vitellogenesis up to 2 weeks after ovulation, its potency increased with the gonad development. The GnRH-stimulated GtH I secretion was decreased in the presence of inhibin in immature fish and at the beginning of vitellogenesis, the GnRH stimulated-release of the GtH II was potentiated at the same stages. In the later stages, inhibin failed to modify the sGnRH-stimulated GtH I and GtH II secretion.

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