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In vitro control of Japanese eel spermatozoa motility by manipulation of the environmental ionic concentration
Ohta, H.; Kagawa, H.; Tanaka, H.; Unuma, T. (2000). In vitro control of Japanese eel spermatozoa motility by manipulation of the environmental ionic concentration, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 415
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Ohta, H.
  • Kagawa, H.
  • Tanaka, H.
  • Unuma, T.
Abstract
    The percent motility of spermatozoa when diluted with 450 mM NaCl increased or decreased with K super(+) and HCO sub(3) super(-) ion concentrations in the isotonic incubation medium. Acquisition and loss of the potential for motility could be induced several times by varying the medium K super(+) and HCO sub(3) super(-) ion concentration. Percent motility of demembranated spermatozoa increased with increasing K super(+) concentration and raised pH of activating solution. Thus, the high extracellular concentrations of both ions are important to raise intracellular K super(+) concentration and intracellular pH under hyperosmotic conditions.
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