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The use of the three-spined stickleback as a potential biomarker for androgenic xenobiotics
Katsiadaki, I.; Scott, A.P.; Matthiessen, P. (2000). The use of the three-spined stickleback as a potential biomarker for androgenic xenobiotics, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 359-361
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Katsiadaki, I.
  • Scott, A.P.
  • Matthiessen, P.
Abstract
    Androgens were administered to sticklebacks via the ambient water. Kidney epithelium height (KEH) measurements indicated significant stimulation in both males and females. Furthermore, the androgen-induced 'glue' protein, spiggin, was localised histochemically in the tubules of the secondary proximal segment. Spiggin was collected from the urinary bladders of fish in breeding conditions, was purified with SDS-PAGE and injected into rabbits. The first stages of ELISA development are promising.
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