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Expression of two estrogen receptors during induced sex reversal in seabream, Sparus aurata
Socorro, S.; Power, D.P.; Canario, A.V.M. (2000). Expression of two estrogen receptors during induced sex reversal in seabream, Sparus aurata, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 237-240
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Socorro, S.
  • Power, D.P.
  • Canario, A.V.M.
Abstract
    The seabream, Sparus aurata, is a protandrous hermaphrodite developing a testis during their first year of life and undergoing sex reversal to female between the second and the third years. Estradiol-17 beta (E sub(2)) has been identified as a mediator of gonadal feminisation in fish. In order to study the mechanism of E sub(2) action in seabream sex reversal, cDNAs encoding two different forms of seabream estrogen receptor (sbER) have been cloned. On the basis of sequence similarity one form of ER is homologous to the classical ER alpha while the other is more related to the newly discovered ER beta . Tissue expression of each sbER revealed some important differences, with ER alpha being detected mainly in testis and ER beta co-expressed in testis and ovary. In an experiment to study the expression of the two receptors, sex reversal was induced by E sub(2). The expression of the two seabream ERs during induced sex reversal was analysed by RT-PCR.
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