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Supercooling of rainbow trout (Oncorhynchus mykiss) eggs at different stages of development
Steinberg, H.; Schmidt-Baulain, R.; Wildenhayn, N.; Holtz, W. (2000). Supercooling of rainbow trout (Oncorhynchus mykiss) eggs at different stages of development, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 432
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer

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  • Steinberg, H.
  • Schmidt-Baulain, R.
  • Wildenhayn, N.
  • Holtz, W.

Abstract
    Rainbow trout embryos at various stages of development were supercooled with various cryoprotectants for 24h. The best hatching rates were achieved with early (190d degree ) eyed eggs suspended in water. With other stages addition of CMC or DMSO proved to be favorable. Fry was obtained after 32 days of storage with 1% CMC (15%) or 5% DMSO (2%).

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