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Effect of cooling rates on the motility and fertility of two sturgeon species sperm after cryopreservation
Trukshin, I.S. (2000). Effect of cooling rates on the motility and fertility of two sturgeon species sperm after cryopreservation, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 417
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Trukshin, I.S., correspondent
Abstract
    Sperm samples from two sturgeon species of Volga river population were cryopreserved by different cooling rates (4 and 10 degree C/min) with the contained 37,1% (weight) sucrose and 5,7% DMSO in the tris-HCl buffer (pH=7,96) cryoprotectant and applied to eggs insemination after thaw. In results the postthaw sperm motility varied from 40% up to 50% in all trials, but only the cooled by 4 degree C/min sperm was able to initiated the normal eggs development.
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