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Isolation and characterization of rainbow trout vasa-like gene and its application to primordial germ cell's marker
Yoshizaki, G.; Sakatani, S.; Tominaga, H.; Takeuchi, Y.; Takeuchi, T. (2000). Isolation and characterization of rainbow trout vasa-like gene and its application to primordial germ cell's marker, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 260
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Yoshizaki, G.
  • Sakatani, S.
  • Tominaga, H.
  • Takeuchi, Y.
  • Takeuchi, T.
Abstract
    The rainbow trout vasa-like gene (RtVLG) and its cDNA were cloned and characterized. RtVLG was predominantly expressed in germ cells, including primordial germ cells (PGCs). Transgenic trout carrying green fluorescent protein gene driven by 5'- and 3'-flanking regions of RtVLG showed fluorescence in PGCs. This transgenic trout can be a useful tool to isolate live PGCs.
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