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Medaka (Oryzias latipes) FTZ-F1 potentially regulates the transcription of P-450 aromatase in ovarian follicles: cDNA cloning and functional characterization
Watanabe, M.; Tanaka, M.; Kobayashi, D.; Oba, Y.; Yoshiura, Y.; Nagahama, Y. (2000). Medaka (Oryzias latipes) FTZ-F1 potentially regulates the transcription of P-450 aromatase in ovarian follicles: cDNA cloning and functional characterization, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 206
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Watanabe, M.
  • Tanaka, M.
  • Kobayashi, D.
  • Oba, Y.
  • Yoshiura, Y.
  • Nagahama, Y.
Abstract
    Our previous findings suggest that cytochrome P-450 aromatase (P-450arom) activity in the ovarian follicle of medaka (Oryzias latipes) is regulated at the transcriptional level. Promoter analysis of the medaka P-450arom gene identified putative FTZ-F1 binding sites. In this study, we cloned a full length cDNA for medaka FTZ-Fl (mdFTZ-F1). The results of gel shift assays, RNase protection assays and transfection assays suggest that mdFTZ-F1 is present in medaka follicles and a change in its binding capacity is the predominant mechanism regulating P-450arom in the ovary.
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