Comparison of mRNA levels of ovarian steroidogenic enzymes between artificially maturing Japanese and naturally maturing New Zealand eels
Matsubara, H.; Kazeto, Y.; Lokman, P.M.; Okumura, H.; Ijiri, S.; Young, G.; Adachi, S.; Yamauchi, K. (2000). Comparison of mRNA levels of ovarian steroidogenic enzymes between artificially maturing Japanese and naturally maturing New Zealand eels, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 181
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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| Auteurs | | Top |
- Matsubara, H.
- Kazeto, Y.
- Lokman, P.M.
- Okumura, H.
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- Ijiri, S.
- Young, G.
- Adachi, S.
- Yamauchi, K.
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| Abstract |
To assess whether regulation of steroidogenesis during artificial maturation resembles that in the wild, we compared mRNA levels of three ovarian steroidogenic enzymes (P450 17 alpha -hydroxylase C17-20 lyase (P450c17), 17 beta -hydroxysteroid dehydrogenase Type I, and aromatase) in artificially maturing Japanese eels (Anguilla japonica; JE) and naturally maturing New Zealand longfinned eels (A. dieffenbachii; LFE). Using northern blot analysis, transcripts for all three enzymes were detected in mid-vitellogenic (MV) ovaries of artificially maturing JE. However, transcripts were almost undetectable in previtellogenic (PV) ovaries of JE, and in both pre and mid-vitellogenic ovaries of wild LFE. Using newly established, high-sensitivity competitive PCR, relative P450c17 transcript levels in artificially maturing JE were about five times higher than in the remaining groups. These findings suggest that steroidogenic enzyme genes are overexpressed during artificial maturation of JE, possibly due to the large quantities of hormone injected. |
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