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Transgenic rainbow trout expressing GnRH antisense mRNA under the control of sGnRH Pab promoter of Atlantic salmon
Uzbekova, S.; Chyb, J.; Alestrom, P.; Prunet, P.; Breton, B. (2000). Transgenic rainbow trout expressing GnRH antisense mRNA under the control of sGnRH Pab promoter of Atlantic salmon, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 68
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., meer
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , meer
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  • Uzbekova, S.
  • Chyb, J.
  • Alestrom, P.
  • Prunet, P.
  • Breton, B.
Abstract
    Recombinant vector containing antisense DNA complementary to Atlantic salmon (Salmo salar) sGnRH cDNA driven by specific promoter Pab derived from sGnRH gene was introduced into rainbow trout (Oncorhynchus mykiss) eggs and resulted in transgenic animals that have integrated few copies of transgene in their genome and can transmit it through germlines. Expression of sGnRH antisense mRNA was detected in the brains of transgenic fish. F1 and F2 pABGnRH-antisense population exhibits high variability of physiological responses, including retardation of spermiation and decrease of GnRH content and gonadotropin level only in sGnRH-antisense expressing (AS+) fish. Expression of sGnRH antisense RNA is not sufficient for complete blocking of sGnRH synthesis in transgenic rainbow trout and to make them sterile.
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