Simple detection method for distinguishing dead and living yeast colonies
Kucsera, J.; Yarita, K.; Takeo, K. (2000). Simple detection method for distinguishing dead and living yeast colonies. J. microbiol. methods 41(1): 19-21. https://dx.doi.org/10.1016/s0167-7012(00)00136-6
In: Journal of Microbiological Methods. Elsevier: Amsterdam. ISSN 0167-7012; e-ISSN 1872-8359, meer
A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5–10 μmol l−1 were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies.
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