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Multi-species transcriptomics reveals evolutionary diversity in the mechanisms regulating shrimp tail muscle excitation-contraction coupling
Huerlimann, R.; Maes, G.E.; Maxwell, M.J.; Mobli, M.; Launikonis, B.S.; Jerry, D.R.; Wade, N.M. (2020). Multi-species transcriptomics reveals evolutionary diversity in the mechanisms regulating shrimp tail muscle excitation-contraction coupling. Gene 752: 144765. https://hdl.handle.net/10.1016/j.gene.2020.144765
In: Gene. ELSEVIER SCIENCE BV: Tokyo; Oxford; New York; Lausanne; Shannon; Amsterdam. ISSN 0378-1119; e-ISSN 1879-0038, meer
Peer reviewed article  
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Trefwoorden
    Penaeidae Rafinesque, 1815 [WoRMS]
    Marien/Kust
Author keywords
    RNA-seq; Penaeid prawn; Ryanodine Receptor; Sarcoplasmic/endoplasmicreticulum Ca2+; ATPase; Voltage gated Calcium channel
Auteurs  Top 
  • Huerlimann, R.
  • Maes, G.E., meer
  • Maxwell, M.J.
  • Mobli, M.
  • Launikonis, B.S.
  • Jerry, D.R.
  • Wade, N.M.
Abstract
    The natural flight response in shrimp is powered by rapid contractions of the abdominal muscle fibres to propel themselves backwards away from perceived danger. This muscle contraction is dependent on repetitive depolarization of muscle plasma membrane, triggering tightly spaced cytoplasmic [Ca2+] transients and rapidly rising tetanic force responses. To achieve such high amplitude and high frequency of Ca2+ transients requires a high abundance of sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA) to rapidly clear cytoplasmic Ca2+ between each transient and an efficient Ca2+ release system consisting of the Ryanodine Receptor (RyR), and voltage gated Ca2+ channels (CaVs). With the aim to expand our knowledge of muscle gene function and identify orthologous genes regulating muscle excitation-contraction (EC) coupling, this study assembled nine Penaeid shrimp muscle transcriptomes. On average, the nine transcriptomes contained 27,000 contigs, with an annotation rate of 36% and a BUSCO completeness of 70%. Despite maintaining their function, the crustacean RyR and CaV proteins showed evidence of significant diversification from mammalian orthologs, while SERCA remained more conserved. Several key components of protein interaction were conserved, while others showed distinct crustacean specific evolutionary adaptations. Lastly, this study revealed approximately 1,000 orthologous genes involved in muscle specific processes present across all nine species.
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