A new Iq-peptide of the Kunitz type from the Heteractis magnifica sea anemone exhibits neuroprotective activity in a model of Alzheimer's disease
Kvetkina, A.N.; Leychenko, E.V.; Yurchenko, E.A.; Pislyagin, E.A.; Peigneur, S.; Tytgat, Y.; Isaeva, M.P.; Aminin, D.L.; Kozlovskaya, E.P. (2018). A new Iq-peptide of the Kunitz type from the Heteractis magnifica sea anemone exhibits neuroprotective activity in a model of Alzheimer's disease. Russian Journal of Bioorganic Chemistry 44(4): 416-423. https://dx.doi.org/10.1134/S106816201804012X
In: Russian Journal of Bioorganic Chemistry. MAIK NAUKA/INTERPERIODICA/SPRINGER: New York. ISSN 1068-1620; e-ISSN 1608-330X, meer
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| Trefwoorden |
Heteractis magnifica (Quoy & Gaimard, 1833) [WoRMS]
Marien/Kust |
| Author keywords |
sea anemone; Kunitz-type protease inhibitors; neuroprotective activity |
| Auteurs | | Top |
- Kvetkina, A.N.
- Leychenko, E.V.
- Yurchenko, E.A.
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- Pislyagin, E.A.
- Peigneur, S., meer
- Tytgat, Y.
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- Isaeva, M.P.
- Aminin, D.L.
- Kozlovskaya, E.P.
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| Abstract |
The HMIQ3c1 recombinant peptide (6434 Da), the Kunitz-type protease inhibitor, which belonged to the new group of the IQ-peptides from the Heteractis magnifica sea anemone was prepared. The inhibitory constant of trypsin by this peptide proved to be 5.0 × 10–8 M. rHMIQ3c1 was shown to have no interaction with eight isoforms of Kv1 channels. This peptide exhibited neuroprotective activity in a concentration of 10 μM, and the peptide increased the viability of cells of the Neuro2a murine neuroblastoma by 39.4 ± 6.6% in the presence of β-amyloid. However, the peptide did not influence the viability of the cells during their incubation with 6-hydroxydofamine. |
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