IMIS | Vlaams Instituut voor de Zee
 

Vlaams Instituut voor de Zee

Platform voor marien onderzoek

IMIS

Publicaties | Instituten | Personen | Datasets | Projecten | Kaarten
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina

Novel nuclear barcode regions for the identification of flatfish species
Paracchini, V.; Petrillo, M.; Lievens, A.; Gallardo, A.P.; Hofherr, J.; Maquet, A.; Silva, A.P.B.; Kagkli, D.M.; Querci, M.; Patak, A.; Angers-Loustau, A. (2017). Novel nuclear barcode regions for the identification of flatfish species. Food Control 79: 297-308. https://dx.doi.org/10.1016/j.foodcont.2017.04.009
In: Food Control. Butterworth Scientific/Elsevier: London. ISSN 0956-7135; e-ISSN 1873-7129, meer
Peer reviewed article  

Beschikbaar in  Auteurs 

Trefwoord
    Marien/Kust
Author keywords
    Bioinformatics; DNA barcoding; Next-generation sequencing; Seafoodidentification

Auteurs  Top 
  • Paracchini, V.
  • Petrillo, M.
  • Lievens, A.
  • Gallardo, A.P.
  • Hofherr, J.
  • Maquet, A.
  • Silva, A.P.B.
  • Kagkli, D.M.
  • Querci, M.
  • Patak, A.
  • Angers-Loustau, A.

Abstract
    The development of an efficient seafood traceability framework is crucial for the management of sustainable fisheries and the monitoring of potential substitution fraud across the food chain. Recent studies have shown the potential of DNA barcoding methods in this framework, with most of the efforts focusing on using mitochondrial targets such as the cytochrome oxidase 1 and cytochrome b genes. In this article, we show the identification of novel targets in the nuclear genome, and their associated primers, to be used for the efficient identification of flatfishes of the Pleuronectidae family. In addition, different in silico methods are described to generate a dataset of barcode reference sequences from the ever-growing wealth of publicly available sequence information, replacing, where possible, labour-intensive laboratory work. The short amplicon lengths render the analysis of these new barcode target regions ideally suited to next-generation sequencing techniques, allowing characterisation of multiple fish species in mixed and processed samples. Their location in the nucleus also improves currently used methods by allowing the identification of hybrid individuals.

Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs