Publicaties | Instituten | Personen | Datasets | Projecten | Kaarten | Infrastructuur
[ meld een fout in dit record ]mandje (0): toevoegen | toon Print deze pagina
Glycerol-3-phosphatase and the control of glycerol metabolism in Dunaliella tertiolecta cells
Belmans, D.; Van Laere, A. (1988). Glycerol-3-phosphatase and the control of glycerol metabolism in Dunaliella tertiolecta cells. Arch. Microbiol. 150(2): 109-112. https://dx.doi.org/10.1007/BF00425148
In: Archives of Microbiology. Springer: Heidelberg; Berlin. ISSN 0302-8933; e-ISSN 1432-072X, meer
Peer reviewed article  
Beschikbaar in  Auteurs 
Trefwoorden
    Dunaliella tertiolecta Butcher, 1959 [WoRMS]
    Marien/Kust
Auteurs  Top 
  • Belmans, D.
  • Van Laere, A.
Abstract
    Glycerol-3-phosphatase (EC 3.1.3.2.1) was studied by following the release of radioactive glycerol from L-(U-14C)glycerol-3-phosphate in Dunaliella tertiolecta enzyme extracts. The reaction showed a neutral pH optimum and had an absolute requirement for Mg2+. The substrate saturation curve was hyperbolic with an apparent Km value for glycerol-3-phosphate of 0.7 mM in the absence of phosphate. Inorganic orthophosphate was a competitive inhibitor of the enzyme with an estimated Kj of 0.1 mM. The glycerol-3-phosphatase reaction was blocked nearly completely by millimolar Ca2+ concentrations. Ca2+ inhibition did not depend on the presence of calmodulin in the reaction medium. The characteristics of glycerol-3-phosphatase are discussed in relation to the regulation of the cyclic glycerol metabolism in Dunaliella cells during periods of osmotic stress.
Alle informatie in het Integrated Marine Information System (IMIS) valt onder het VLIZ Privacy beleid Top | Auteurs