Preparation of bromine-77 labeled proteins using bromoperoxidase
In: Applied Radiation and Isotopes. Elsevier: Amsterdam. ISSN 0969-8043; e-ISSN 1872-9800, meer
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| Abstract |
The halogenating enzyme bromoperoxidase (BPO, EC 1.11.1.7), isolated from the brown algae Ascophyllum nodosum, was used to catalyze the radiobromination of an anti-hPLAP monoclonal antibody. Radiobromination of bovine serum albumin was also performed and used as control method. The proteins are labeled with bromine-77, produced by the 75As (α, 2n) 77Br reaction.The essential reaction parameters, such as concentration of protein and hydrogen peroxide, the amount of bromoperoxidase, the pH of the reaction mixture, the reaction time and temperature were optimized.The immunoreactivity of the radiolabeled monoclonal antibody was checked with a “Sandwich” ELISA. |
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