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High performance liquid chromatographic determination of free amino acids in algae
Péter, A.; Tourwè, D.; Baumann, E.M.; Elskens, M.; Goeyens, L. (1999). High performance liquid chromatographic determination of free amino acids in algae. Journal of Liquid Chromatography & Related Technologies 22(7): 1077-1093. dx.doi.org/10.1081/JLC-100101719
In: Journal of Liquid Chromatography & Related Technologies. TAYLOR & FRANCIS INC: Philadelphia. ISSN 1082-6076; e-ISSN 1520-572X, meer
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  • Péter, A.
  • Tourwè, D.
  • Baumann, E.M.
Abstract
    The amino acid compositions were determined in four different algal species: the temperate Thalassiosira rotula and Phaeocystis globosa and the polar Thalassiosira antarctica and Phaeocystis antarctica.An extraction procedure was developed to obtain amino acids from phytoplankton species. The amino acids were analysed after precolumn derivatization with 9-fluorenylmethoxycarbonyl chloride (FMOC-Cl); the procedure was optimized and the samples were analysed quantitatively on a Superspher RP8 C8 column. The good stability of the FMOC derivatives was utilized to develop a new semipreparative chromatographic method for the separation and collection of the most important amino acids.
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