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Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm
Vandecandelaere, I.; Nercessian, O.; Segaert, E.; Achouak, W.; Faimali, M.; Vandamme, P. (2008). Ruegeria scottomollicae sp. nov., isolated from a marine electroactive biofilm. Int. J. Syst. Evol. Microbiol. 58: 2726-2733. dx.doi.org/10.1099/ijs.0.65843-0
In: International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology: Reading. ISSN 1466-5026; e-ISSN 1466-5034, meer
Peer reviewed article  

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  • Vandecandelaere, I., meer
  • Nercessian, O.
  • Segaert, E., meer
  • Achouak, W.
  • Faimali, M.
  • Vandamme, P., meer

Abstract
    Seventy isolates were obtained from a marine electroactive biofilm that was generated on a cathodically polarized stainless steel electrode (Genoa, Italy). The genetic diversity was investigated by means of BOX-PCR fingerprinting and two clusters of isolates with similar BOX-PCR profiles were delineated. Whole-cell fatty acid methyl ester analysis and 16S rRNA gene sequence analysis showed that the isolates belonged to the Roseobacter lineage of the class Alphaproteobacteria. DNA–DNA hybridization experiments and a biochemical analysis demonstrated that four isolates belonged to the species Ruegeria mobilis. However, 66 isolates from the second BOX-PCR cluster constituted a novel species within the genus Ruegeria, for which the name Ruegeria scottomollicae sp. nov. is proposed. The DNA G+C content was 61.0±0.4?%. The type strain is LMG 24367T (=CCUG 55858T).

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