PCBs do not induce DNA breakage in vitro in human lymphocytes
Belpaeme, K.; Delbeke, K.; Zhu, L.; Kirsch-Volders, M. (1996). PCBs do not induce DNA breakage in vitro in human lymphocytes. Mutagenesis 11(4): 383-389
In: Mutagenesis. United Kingdom Environmental Mutagen Society: Oxford. ISSN 0267-8357; e-ISSN 1464-3804, meer
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| Auteurs | | Top |
- Belpaeme, K., meer
- Delbeke, K., meer
- Zhu, L.
- Kirsch-Volders, M.
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| Abstract |
Polychlorinated biphenyls (PCBs) are classified by IARC as non-mutagenic in vivo, However, despite almost 20 years of research, their mutagenicity in vitro is still debatable. In this work the in vitro cytochalasin B micronucleus test and the alkaline comet assay applied to human lymphocytes were used to study the genotoxicity of a PCB, PCB77, at concentrations ranging from 0.01 to 100 µg/ml, was used in whole blood or isolated lymphocyte cultures, with final dimethylsulfoxide percentages of 0.5-2%. In the micronucleus test lymphocytes were exposed for 48 h, and in the alkaline comet assay for 30 min, 1 h and 3 h. No increases of single strand breaks or micronucleus frequencies was found, in contrast to previously reported data, Our data indicate that PCB77 has no clastogenic properties in human lymphocytes. |
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