Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka
Abeysinghe, P.D.; Triest, L.; De Greef, B.; Koedam, N.; Hettiarachi, S. (1999). Genetic differentiation between Bruguiera gymnorhiza and B. sexangula in Sri Lanka. Hydrobiologia 413: 11-16. https://dx.doi.org/10.1023/A:1003899028558
In: Hydrobiologia. Springer: The Hague. ISSN 0018-8158; e-ISSN 1573-5117, meer
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| Trefwoorden |
Acids > Organic compounds > Organic acids > Nucleic acids > DNA
Biology > Genetics > Population genetics
Distribution > Geographical distribution
Genetic parameters > Genetic variance
Markers > Genetic markers > Dna > Random amplified polymorphic dna
Natural populations > Plant populations
Polymerase chain reaction
Subpopulations
Water bodies > Inland waters > Wetlands > Swamps > Mangrove swamps
Bruguiera gymnorhiza (L.) Lamk. [WoRMS]; Bruguiera sexangula (Lour.) Poir. [WoRMS]
Marien/Kust; Brak water |
| Auteurs | | Top |
- Abeysinghe, P.D., meer
- Triest, L., meer
- De Greef, B.
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- Koedam, N., meer
- Hettiarachi, S.
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| Abstract |
The identification of populations of Bruguiera sexangula, Bruguiera gymnorhiza and their putative hybrids in the field is difficult using only morphological and phenological characters. Using a PCR based technique, RAPD (Random Amplified Polymorphic DNA), the genetic variation of Bruguiera populations was studied from contrasting climatic and geographic regions along the southwest coastal region of Sri Lanka. Out of 45 primers screened, 20 primers allowed us to observe polymorphism, not only between species (interspecific) but also within the species (intraspecific). Analysis of RAPD data appears to be helpful in determining the genetic relationship among populations of B. gymnorhiza and B. sexangula. RAPD markers revealed that the two species are well separated without any hybrid position between the two taxa though they occur in mixed stands. Although sampling sizes of populations of this study were small, genetic variation among B. gymnorhiza and B. sexangula populations could be observed. For B. sexangula, it was possible to differentiate each of the three populations, even when using a small number of primers. |
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