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Functional and molecular aspects of the gonadrotopin releasing hormones in the African catfish, Clarias gariepinus
Goos, H.; Bosma, P.; Zandbergen, T.; Schulz, R.; Bogerd, J. (1995). Functional and molecular aspects of the gonadrotopin releasing hormones in the African catfish, Clarias gariepinus. Neth. J. Zool. 45(1-2): 147-151
In: Netherlands Journal of Zoology. E.J. Brill: Leiden. ISSN 0028-2960; e-ISSN 1568-542X, meer
Peer reviewed article  
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  • Goos, H.
  • Bosma, P.
  • Zandbergen, T.
  • Schulz, R.
  • Bogerd, J.
Abstract
    Two molecular forms of GnRH were identified in the Afrcan catfish, Clarias Gariepinus: catfish gonadotropin-releasing hormone (cGnRH-II). They differ in gonadotropine hormone (GTH)-release activity, cGnRH-II being about a hundred times as potent as cfGnRH. This difference corresponds to different GnRH receptor binding affinities. cDNAs encoding both GnRH preprohormones were cloned and specific antibodies were raised against the GnRHs. By immunocytochemistry and in in situ hybridization cGnRH-II was localised in the midbrain tegmentum, cfGnRH in the rostro-ventral forebrain.
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