Comparison of methods for the determination of chlorophyll in estuarine sediments
In: Netherlands Journal of Sea Research. Netherlands Institute for Sea Research (NIOZ): Groningen; Den Burg. ISSN 0077-7579; e-ISSN 1873-1406, meer
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| Abstract |
Five methods for the determination of the chlorophyll content in solutions of pure chlorophyll and pigment extracts of estuarine sediments were compared. If only chlorophyll a was present, fluorometric, spectrophotometric as well as HPLC-methods yielded reliable results. In samples containing a mixture of pigments, the spectrophotometric method appears to be preferable to the fluorometric determination, unless the relation between spectrophotometer and fluorometer readings is checked regularly. However, in pigment extracts that contain a mixture of pigments, the most reliable results will be obtained with the HPLC-technique because interference by the various pigments in the extract is excluded as they are separated before quantification. Similar results are reported from the literature for phytoplankton. The conclusions drawn from the analysis of samples from the water column are thus also valid for sediments containing chlorophyll in addition to large amounts of organic matter. |
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