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Factors affecting survival of cryopreserved rotifers (Branchionus plicatilis O.F. Műller)
Hadani, A.; Beddig, S.; Lubzens, E. (1992). Factors affecting survival of cryopreserved rotifers (Branchionus plicatilis O.F. Műller), in: Progress in aquaculture research: proceedings of the 4th German-Isreali Status Seminar held on October 30-31, 1990. Spec. Publ. Eur. Aquacult. Soc, 17: pp. 253-267
In: Moav, B.; Hilge, V.; Rosenthal, H. (Ed.) (1992). Progress in aquaculture research: Proceedings of the 4th Status Seminar held on October 30-31, 1990 in GKSS-Forschungszentrum Geesthacht GmbH. Spec. Publ. Eur. Aquacult. Soc, 17. European Aquaculture Society: Oostende. ISBN 90-71625-11-7. 360 pp., meer
In: Spec. Publ. Eur. Aquacult. Soc., meer
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  • Hadani, A.
  • Beddig, S.
  • Lubzens, E.
Abstract
    Rotifer amictic egs survived cooling to -30°C (up to 38,9%) or -196°C (up to 15%). The type of cryoprotectant and its concentration affected the survival of eggs exposed to subfreezing temperatures. However, a treatment that led to high survival at -30°C didn't necessarily ensure high survival at -196°C. An increase in the number of surviving eggs was obtained by using ovigorous females from a mixed age population. Incubating rotifers with the cryoprotectant at room temperature for 30-40 min. before cooling increased the chances of survival after exposure to -196°C. Gradual dillution of the cryoprotectant following thawing slightly improved the survival of eggs cooled to -30°C. Rotifers cultured on Nannochloropsis showed more consistent results after cooling to -196°C than those cultured on Isochrysis.
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