Effects of bistheonellide A, an actin-polymerization inhibitor, on Chinese hamster V79 cells and on IL-8 production in PMA-stimulated HL-60 cells
Oda, T.; Xu, J.; Fujita, A.; Mochizuki, M.; Namikoshi, M. (2006). Effects of bistheonellide A, an actin-polymerization inhibitor, on Chinese hamster V79 cells and on IL-8 production in PMA-stimulated HL-60 cells. Mar. Drugs 4(1): 22-27. https://dx.doi.org/10.3390/md401022
In: Marine Drugs. Molecular Diversity Preservation International (MDPI): Basel. ISSN 1660-3397; e-ISSN 1660-3397, meer
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| Auteurs | | Top |
- Oda, T.
- Xu, J.
- Fujita, A.
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- Mochizuki, M.
- Namikoshi, M.
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| Abstract |
Bioassay-guided isolation from the ethanol extract of a marine sponge Theonella sp. collected in Palau yielded bistheonellide A, which strongly inhibited the colony formation of Chinese hamster V79 cells (EC50 = 6.8 nM). Bistheonellide A is an actinpolymerization inhibitor and was suggested to control cytokine production. Therefore, we attempted to detect an effect of this compound on IL-8 production in PMA-stimulated HL-60 cells. Interestingly, bistheonellide A did not modulate the production of IL-8 under cytotoxic concentrations as determined by LDH analysis. Although the correlation between the inhibition of microtubule assembly and the stimulation of IL-8 production has been observed for several compounds, the polymerization of actin was not related to an IL-8 production in the case of bistheonellide A. It will be suggested that the actin polymerization is not involved in the IL-8 production system. |
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