Measurement of sperm motility in trout and carp
Billard, R.; Cosson, M.P. (1989). Measurement of sperm motility in trout and carp, in: De Pauw, N. et al. (Ed.) Aquaculture: a biotechnology in progress: volume 1. pp. 499-503
In: De Pauw, N. et al. (1989). Aquaculture: a biotechnology in progress: volume 1. European Aquaculture Society: Bredene. ISBN 90-71625-03-6. 1-592 pp., meer
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Documenttype: Congresbijdrage
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| Abstract |
Spermatozoa of teleost fish with external fertilization are only motile for 30s to a few minutes. They become motile when released in the ambient medium, whether it be fresh- or seawater. The spermatozoa of trout are immobilized in the seminal fluid due to the presence of K+ (10 to 40mM) and those of carp are immobilized due to osmotic pressure (200mOsm). Trout spermatozoa, diluted in a solution of NaCl125mM and Tris 20mM of pH 9.0, are only motile tor 20 to 30s (active swimming with forward movement), after which most of them stop moving. The characteristics of sperm movement vary during the period of motility. The flagellar beating frequency, measured stroboscopically and the speed of movement are about 60 Hz and more than 250um.s-1, respectively, during dilution; these rates decrease gradually to 10 to 20Hz and 20 to 25um.s-1, 25s later at which time most of the spermatozoa stop swimming. When 1mM Ca++ is added to the diluent, motility is prolonged beyond 30s. Carp spermatozoa diluted in freshwater, move actively for less than 1 min. The flagellar beating frequency is higher than 50 Hz immediately after dilution and declines rapidly to reach 10Hz, 40-50s later. The decline in beating frequency parallels the decrease in fertilizing capacity of the spermatozoa after dilution for both trout and carp. |
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