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Immunostimulatory effects of marine bacteria and bacterial endotoxin in sea spray aerosols
Citatie
Schütte, W., Li, Y., De Rijcke, M. (2023): Immunostimulatory effects of marine bacteria and bacterial endotoxin in sea spray aerosols. Flanders Marine Institute (VLIZ). https://marineinfo.org/id/dataset/8344
Contact:
De Rijcke, Maarten ;
Beschikbaarheid: De data kan beschikbaar gesteld worden op aanvraag via de hogervermelde contactgegevens.
Beschrijving
Bacterial counts and endotoxin concentrations of aerosol samples collected at the beach of Ostend, Belgium, and generated in the laboratory with a marine aerosol referenc tank (MART). Data regarding gene induction in human reporter cell lines (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6, THP1-Dual) after stimulation with these collected aerosols. meerThe data was used to examine levels of bacterial endotoxins in SSA and their effect on cell-signalling pathways of the human innate immune system. Human reporter cell lines (HEK-Blue hTLR4, HEK-Blue hTLR2-hTLR6 and THP1-Dual) were exposed to aerosol samples collected at the beach of Ostend (Belgium) and generated with a Marine Aerosol Reference Tank (MART) in the laboratory. Bacteria cell count s in aerosol samples were measured with flow cytometry and endotoxins were quantified using the rFC assay. Scope Kernwoorden: , Immunology, Mariene ecologie, Sea spray, ANE, België, Oostende Geografische spreiding ANE, België, Oostende [Marine Regions] Spreiding in de tijd
3 Maart 2023 - 26 Mei 2023 Parameters
Endotocin concentration: rF C assay rF C assay Count of bacteria: flowcytometrie Bijdrage door
Dataset status: Afgelopen
Data type: Data
Data oorsprong: Onderzoek: experiment
Metadatarecord aangemaakt: 2023-06-02
Informatie laatst gewijzigd: 2023-06-02
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