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Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak
Citatie
Rahlff, J.; Esser, S.P.; Heinrichs, M.E.; Giebel, HA.; Probst, A.J.; (2023): Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak. PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.947348
Beschikbaarheid:
 Deze dataset valt onder een Creative Commons Naamsvermelding-NietCommercieel-GeenAfgeleideWerken 4.0 Internationaal-licentie.Beschrijving
Flow cytometry samples for analysis of virus-like particle, prokaryote and small phototrophic eukaryote abundances were collected from sea foam, surface microlayer and 1-m deep water from the Swedish Skagerrak during a 1-month campaign at Tjärnö Marine Laboratory in February 2020. meer
Using a small boat, foam and microlayer were collected using a glass plate sampler, and 1-m deep water using a syringe connected to a hose. In addition, rainwater for flow cytometry was collected close to the coast in Duran bottles. Samples were fixed with glutardialdehyde (1% final concentration, Merck, Sweden), stored for 1 hour in the dark and subsequently stored at -80 °C. The purpose of the cellular and viral abundance assessment was to complement metagenomic analyses to investigate viral dispersal across the air-sea interface and along the natural water cycle. In addition, metadata for light conditions, wind speed, salinity, and temperature were recorded. For further details on the campaign and flow cytometric analysis, please refer to Rahlff et al., 2022. Scope Thema's: Biologie > Pleuston > Neuston Kernwoorden: Marien/Kust, Zoet water, Air-sea, Bacterial abundance, Sea foam, Sea-surface microlayer, Virus-like particles, ANE, Skagerrak Geografische spreiding ANE, Skagerrak [Marine Regions] Spreiding in de tijd
3 Februari 2020 - 26 Februari 2020 Parameters
Virus-like particles: Flow cytometer, BD Biosciences, BD Accuri C6 Eukaryotes, phototrophic, small: Flow cytometer, BD Biosciences, BD Accuri C6 Prokaryotes: Flow cytometer, BD Biosciences, BD Accuri C6 Saliniteit: Thermosalinometer, portable, WTW Multiline 3420 Watertemperatuur: Thermosalinometer, portable, WTW Multiline 3420 Windsnelheid: Anemometer, AN-10, Voltcraft Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, meer
Publicatie
Gebaseerd op deze dataset
Rahlff, J. et al. (2023). Marine viruses disperse bidirectionally along the natural water cycle. Nature Comm. 14(1): 6354. https://dx.doi.org/10.1038/s41467-023-42125-5, meer
Rahlff, J. et al. (2022). Heads in the clouds: marine viruses disperse bidirectionally along the natural water cycle. bioRxiv 2022: 1-35. https://dx.doi.org/10.1101/2022.06.21.497027 , meer
Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2023-05-11
Informatie laatst gewijzigd: 2024-02-05
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