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Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition
Citatie
Bielewicz S, Bell E, Kong W, Froedberg I, Priscu J, Morgan-Kiss R (2021): Protist diversity in a permanently ice-covered Antarctic Lake during the polar night transition. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=protist_diversity_in_antarctic_lake_during_polar_night&v=1.1 https://doi.org/10.15468/t628ps
Contact:
Morgan-Kiss, Rachael Beschikbaarheid: Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.
Nota: The publisher and rights holder of this work is SCAR - Microbial Antarctic Resource System. This work is licensed under a Creative Commons Attribution (CC-BY) 4.0 License.
Beschrijving
Amplicon sequencing dataset targeting eukaryote microorganisms (18S ssu rRNA marker gene) in permanently ice-covered lakes in the McMurdo Dry Valleys (Antarctica). Samples (n=89) were taken in the photic zone of the east lobe (ELB) and west lobe (WLB) of the chemically stratified Lake Bonney. meer
Samples (1 liter) were vacuum concentrated (5 kPa) onto 47 mm 0.45 µm Durapore polyvinylidene fluoride membrane filters (Millipore). The filters were frozen immediately in liquid nitrogen and transported frozen to McMurdo Station, where they were stored at -80 degrees C until DNA extraction. Lake Bonney in the McMurdo Dry Valleys (Antarctica). Samples were collected at 6, 13, 18, and 20m in the east lobe (ELB) and 10, 13, 15, and 20m in the west lobe (WLB) of Lake Bonney from February to April 2008. All collection depths are relative to the piezometric water level in the ice borehole. DNA was isolated from a whole filter using the MP DNA kit (MP Biomedicals, CA) following the kit’s instruction. Sequencing was performed using the BigDye Terminator v3.1 cycle sequencing kit (ABI, CA) with M13R primer and the fragments were sequenced on an Applied Biosystems 3730×l DNA Analyzer (ABI, CA). The 18S rRNA gene sequences reported in the current study have been deposited in GenBank under accession numbers GU969060 to GU969102. Scope Thema's: Biologie > Ecologie - biodiversiteit Kernwoorden: Zoet water, Eukaryotes, Lake ice, Metadata, Micro-organismen, Rrna, Sequencing, Antarctica, Victoria Land, Bonney L., Antarctica, Victoria Land, McMurdo Dry Valleys Geografische spreiding Antarctica, Victoria Land, Bonney L. [Marine Regions] Antarctica, Victoria Land, McMurdo Dry Valleys [Marine Regions] Spreiding in de tijd
18 November 2010 - 15 Maart 2011 Parameters
18S Moleculaire data Bijdrage door
Miami University, meer, data creator
Gerelateerde datasets
Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2021-07-06
Informatie laatst gewijzigd: 2021-07-06
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