IMIS

GRL: GEANS Reference Library

Citatie

Christodoulou M.; Beentjes K.; van der Hoorn B., Van Den Bulcke, L., Derycke S., De Backer, A.; Martinez Arbizu P.; 2021; GEANS Reference Library dx.doi.org/10.5883/DS-GEANS1

Contact: Martinez Arbizu, Pedro ;

Beschikbaarheid: Vrij beschikbaar
The data are freely available to anybody and may be used for any purpose. Usage acknowledgement may be required

Beschrijving

Benthic macroinvertebrates are key components in environmental impact assessments. Nevertheless, their use as bioindicators can be constrained by the time- and cost-consuming processes needed for their morphological identification. The recent advances in high-throughput sequencing, particularly DNA metabarcoding can provide an alternative to morphology-based approaches. The main limitation for DNA-based tools to be implemented in biomonitoring projects is the considerable investment needed to build high-quality and curated taxonomic reference DNA sequence libraries for species identification. To begin addressing this shortage, GEANS project aims in developing a curated DNA reference library based on mitochondrial cytochrome c oxidase subunit I (COI) for the North Sea macrobenthos that will serve as the backbone of all the molecular protocols. meer

For the collection of the macroinvertebrates, targeted sampling campaigntake place across the North Sea Region, while existing collections are being scanned. Macrobenthic specimens are being processed following a series of standardise work flows covering sampling, morphological identifications, molecular lab processing and data handling. All specimens are photographed and together with the accompanying sequences, collection and taxonomic data are archived in a dedicate BOLD project. Voucher specimens and DNA extracts are archived at three different institutes in Germany (Senckenberg am Meer), the Netherlands (Naturalis) and Belgium (ILVO). Once available, this DNA-library will be based on taxonomically well-curated specimens and it will support the implementation of fast, cost-efficient and reliable DNA-based identifications and subsequently environmental health assessments in the North Sea.

Scope

Thema's:

Biologie, Biologie > Benthos, Biologie > Benthos > Macrobenthos

Kernwoorden:

Marien/Kust, Brak water, Biodiversiteit, COI, Dna, DNA barcoding, Genetic, Genetic data, Marien, ANE, Noordzee, Animalia

Geografische spreiding

ANE, Noordzee [Marine Regions]

Spreiding in de tijd

1 Maart 2019 - 28 Februari 2022
Quasi continu (< 1 min.)

Taxonomic coverage

Animalia [WoRMS]

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