| one publication added to basket [280860] | Peptidoglycan recognition proteins with amidase activity in early deuterostornes (Echinodermata)
Coteur, G.; Mellroth, P.; De Lefortery, C.; Gillan, D.; Dubois, P.; Communi, D.; Steiner, H. (2007). Peptidoglycan recognition proteins with amidase activity in early deuterostornes (Echinodermata). Dev. Comp. Immunol. 31(8): 790-804. https://dx.doi.org/10.1016/j.dci.2006.11.006
In: Developmental and Comparative Immunology. Elsevier: New York,. ISSN 0145-305X; e-ISSN 0145-305X, meer
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| Trefwoorden |
Echinodermata [WoRMS]
Marien/Kust |
| Author keywords |
immunity; PGRP; echinoderms; peptidoglycan; reactive oxygen species-;phagocytes; inflammation |
| Auteurs | | Top |
- Coteur, G.
- Mellroth, P.
- De Lefortery, C.
- Gillan, D., meer
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- Dubois, P., meer
- Communi, D.
- Steiner, H.
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| Abstract |
Despite the ecological and evolutionary importance of echinoderms, very little is known about the immune mechanisms in this group especially regarding humoral immunity. In this paper, we screened for proteins putatively involved in immunity in the common European seastar Asterias rubens using a mass spectrometry-based proteomic approach. Two proteins showed striking sequence similarities with peptidoglycan recognition proteins (PGRPs). The two seastar proteins were identified as a single protein, termed PGRP-S1a, occurring in two forms in the coelomic plasma, one of 20 kDa and another of 22 kDa. We also cloned and sequenced a second member of the PGRP family, termed PGRP-S2a. It has a calculated molecular mass of 21.3 kDa and is expressed in circulating phagocytes. Both the S1a-cDNA from coelomic epithelium RNA and the S2a-cDNA from phagocytes code for the amino acid residues necessary for peptidoglycan degradation. PGRP-S1a did not affect the phagocytic activity of seastar immune cells towards Micrococcus luteus but inhibited their production of reactive oxygen species (ROS). A recombinant, His-tagged, PGRP-S2a degrades peptidoglycan and increases the phagocytosis of M. luteus cells by seastar phagocytes. |
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