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Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak
Citatie
Rahlff, J.; Esser, S.P.; Heinrichs, M.E.; Giebel, HA.; Probst, A.J.; (2023): Flow cytometric assessment of virus-like particle, prokaryote and small phototrophic eukaryote abundances from sea foam, surface microlayer, 1-m depth, rainwater from the Swedish Skagerrak. PANGAEA https://doi.pangaea.de/10.1594/PANGAEA.947348


Beschrijving
Flow cytometry samples for analysis of virus-like particle, prokaryote and small phototrophic eukaryote abundances were collected from sea foam, surface microlayer and 1-m deep water from the Swedish Skagerrak during a 1-month campaign at Tjärnö Marine Laboratory in February 2020. meer

Using a small boat, foam and microlayer were collected using a glass plate sampler, and 1-m deep water using a syringe connected to a hose. In addition, rainwater for flow cytometry was collected close to the coast in Duran bottles. Samples were fixed with glutardialdehyde (1% final concentration, Merck, Sweden), stored for 1 hour in the dark and subsequently stored at -80 °C. The purpose of the cellular and viral abundance assessment was to complement metagenomic analyses to investigate viral dispersal across the air-sea interface and along the natural water cycle. In addition, metadata for light conditions, wind speed, salinity, and temperature were recorded. For further details on the campaign and flow cytometric analysis, please refer to Rahlff et al., 2022.

Scope
Thema's:
Biologie > Pleuston > Neuston
Kernwoorden:
Marien/Kust, Zoet water, Air-sea, Bacterial abundance, Sea foam, Sea-surface microlayer, Virus-like particles, ANE, Skagerrak

Geografische spreiding
ANE, Skagerrak [Marine Regions]

Spreiding in de tijd
3 Februari 2020 - 26 Februari 2020

Parameters
Virus-like particles Methode
Eukaryotes, phototrophic, small Methode
Prokaryotes Methode
Saliniteit Methode
Watertemperatuur Methode
Windsnelheid Methode
Virus-like particles: Flow cytometer, BD Biosciences, BD Accuri C6
Eukaryotes, phototrophic, small: Flow cytometer, BD Biosciences, BD Accuri C6
Prokaryotes: Flow cytometer, BD Biosciences, BD Accuri C6
Saliniteit: Thermosalinometer, portable, WTW Multiline 3420
Watertemperatuur: Thermosalinometer, portable, WTW Multiline 3420
Windsnelheid: Anemometer, AN-10, Voltcraft

Project
ASSEMBLE+: Association of European Marine Biological Laboratories Expanded, meer

Publicatie
Gebaseerd op deze dataset
Rahlff, J. et al. (2023). Marine viruses disperse bidirectionally along the natural water cycle. Nature Comm. 14(1): 6354. https://dx.doi.org/10.1038/s41467-023-42125-5, meer
Rahlff, J. et al. (2022). Heads in the clouds: marine viruses disperse bidirectionally along the natural water cycle. bioRxiv 2022: 1-35. https://dx.doi.org/10.1101/2022.06.21.497027 , meer

Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2023-05-11
Informatie laatst gewijzigd: 2024-02-05
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