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fumarolic microbial communities at Tramway Ridge, Mt. Erebus, Antarctica
Citatie
Herbold C, Lee C, McDonald I, Cary C (2019): fumarolic microbial communities at Tramway Ridge, Mt. Erebus, Antarctica. v1.2. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=fumarolic_antarctic_microbial_communities_at_mount_erebus&v=1.2 https://doi.org/10.15468/iab9ae
Contact:
Herbold, Craig Beschikbaarheid: Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.
Beschrijving
Microbial (Bacteria) Dataset containing amplicon sequencing samples and metagenome shotgun sequencing samples from two fumarole soil location at Tramway Ridge, Mount Erebus, Antarctica (ASPA 130). meer
All suggested sterilization protocols for entering into this protected site were adhered to, following the ASPA 130 Management Plan ( http://www.scar.org/publications/bulletins/151/aspa130.html). Sites were chosen based on measuring a surface temperature of 65 °C with a stainless steel Checktemp1 temperature probe (Hanna Instruments, Rhode Island, USA), sterilized with 70% ethanol immediately before each use. Surface ‘crust’ was set aside before collecting samples. Samples were collected by aseptically removing the top 2 cm of sediment in an ~25 cm2 area. Sediment was placed into a fresh 50 ml Falcon tube. Sampling continued with the collection of a second (2–4 cm depth) and third (4–8 cm depth) layer following the same procedures. Temperature measurements were repeated for each layer sampled. All samples were immediately frozen, transported back to the University of Waikato frozen and maintained at −80 °C in the laboratory until analysed. Study Extent: Sediment samples were collected within the Tramway Ridge Antarctic Specially Protected Area (ASPA 130) in February 2009 from two sites (site A (active site): 77° 31.103′ S, 167° 6.682′ S and site B (passive site): 77° 31.106′ S, 167° 6.668′ E). Method step description:
Scope Kernwoorden: Terrestrisch, Dna sequencing, Fumaroles, Metadata, Antarctica, Bacteria Geografische spreiding Spreiding in de tijd
Februari 2009 Taxonomic coverage
Bacteria [WoRMS]
Parameter
Moleculaire data Bijdrage door
University of Waikato, meer, data creator
Gerelateerde datasets
Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2019-04-10
Informatie laatst gewijzigd: 2019-04-10
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