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Nanopore based eDNA metabarcoding of marine vertebrates in the North Sea
Citatie
Doorenspleet, K.; Jansen, L.; Oosterbroek, S.; Kamermans, P.; Wurz, E.; Murk, A.; Nijland, R.; Marine Animal Ecology; Wageningen Marine Research; Wageningen University and Research Centre: The Netherlands; (2024): Nanopore based eDNA metabarcoding of marine vertebrates in the North Sea. https://marineinfo.org/id/dataset/8601

Beschikbaarheid: Creative Commons License Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.

Beschrijving
This data is the result of DNA-based water sampling of eDNA in different locations in the North Sea. meer

Molecular Data generation of marine biodiversity data plays a central role in monitoring and better understanding of North Sea marine ecosystems. By applying DNA-based techniques, this study specifically aims to present a metabarcoding pipeline for Nanopore sequencing and compare a newly designed 2kb primer with commonly used MiFish pimer. eDNA samples were taken in The ray reef aquarium in Dolfinarium , The brokum reef ground in the North sea, at sany bottom, Gmini windpark and around 3 shipwrecks in the North Sea and analysed through metabarcoding with nanopore sequencing and results were compared with morphology and commonly used MiFish primer in order to demonstrate the value of applying this technique.

Scope
Thema's:
Biologie > Invertebraten, Biologie > Vis
Kernwoorden:
Marien/Kust, , Elasmobranchs, Fish, Metabarcoding, MinION, ANE, Noordzee

Geografische spreiding
ANE, Noordzee [Marine Regions]

Spreiding in de tijd
1 Juli 2019 - 31 Juli 2020

Parameters
Diepte Methode
pH-zuurtegraad Methode
Saliniteit Methode
Temperatuur Methode
Diepte: Diving computer
pH-zuurtegraad: CTD
CTD type: SBE-19 (www.seabird.com); depth profiles of temperature and salinity equipped with a OBS probe for relative turbidity measurements
Saliniteit: CTD
CTD type: SBE-19 (www.seabird.com); depth profiles of temperature and salinity equipped with a OBS probe for relative turbidity measurements
Temperatuur: CTD
CTD type: SBE-19 (www.seabird.com); depth profiles of temperature and salinity equipped with a OBS probe for relative turbidity measurements

Bijdrage door
Wageningen University and Research Centre; Marine Animal Ecology, meerdata creator
Wageningen University and Research Centre; Wageningen Marine Research (WMR), meerdata creator

Dataset status: Afgelopen
Data type: Data
Data oorsprong: Onderzoek: veldexperiment
Metadatarecord aangemaakt: 2024-06-17
Informatie laatst gewijzigd: 2024-06-27
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