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V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island
Citatie
Chua C, Yong S, Gonzalez M, Lavin P, Cheah Y, Tan G, Wong C (2019): V3-V4 16S rDNA of soil bacteria in King George Island and Deception Island. v1.1. SCAR - Microbial Antarctic Resource System. Dataset/Metadata. https://ipt.biodiversity.aq/resource?r=soil_bacteria_king_george_island_and_deception_island&v=1.1 https://doi.org/10.15468/spzi1j
Contact: Chua, C. ; Wong, C.
Toegang tot data
Gearchiveerde data
Beschikbaarheid: Creative Commons License Deze dataset valt onder een Creative Commons Naamsvermelding 4.0 Internationaal-licentie.
Beschrijving
Amplicon sequencing dataset (Illumina MiSeq 250bp PE) of soil bacteria (16S ssu rRNA gene, v3-v4) on King George Island and Deception Island (Antarctica). meer

Samples were stored at –20°C immediately after sampling.
Study Extent: Soil samples were collected from King George Island (S62°11′35.3′′; W58°56′6.2′′) and Deception Island (S62°59′023′′; W60°40′51.7′′), Antarctica in February 2007.
Method step description:
  1. Total bacterial genomic DNA was extracted from soils using the method described in Zhou et al. (1996) DNA recovery from soils of diverse composition. Appl. Environ. Microbiol., 62(2), 316–322.
  2. The concentration and purity of the extracted genomic DNA were measured using the NanoDropTM 2000 spectrophotometer (Thermo Scientific Inc., Waltham, MA, USA).
  3. The V3–V4 region of the bacterial 16S rRNA gene was amplified using locus-specific primers, S-DBact-0341-b- S-17 (5′-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG CCT ACG GGN GGC WGC AG-3′)and S-D-Bact-0785-a-A-21 (5′-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGA CTA CHV GGG TAT CTA ATC C-3′). The 16S rDNA amplicons were subjected to paired-end sequencing on Illumina MiSeq platform using 2 × 250 bp MiSeq Reagent Kit v2 (Illumina® Inc., San Diego, California).
Scope
Kernwoorden:
Terrestrisch, Dna sequencing, Metadata, Antarctica, South Shetland I., Deception I., Antarctica, South Shetland I., King George I., Bacteria
Geografische spreiding
Antarctica, South Shetland I., Deception I. [Marine Regions]
Antarctica, South Shetland I., King George I. [Marine Regions]
Spreiding in de tijd
Februari 2007
Taxonomic coverage
Bacteria [WoRMS]
Parameter
Moleculaire data
Bijdrage door
Universiti Malaysia Sabah, meer, data creator
Chua, C.
Yong, S.
Wong, C.
Instituto Antártico Chileno (INACH), meer, data creator
Gonzalez, M.
Lavin, P.
University Putra Malaysia, meer, data creator
Cheah, Y.
University of Malaya, meer, data creator
Tan, G.
Gerelateerde datasets
Gepubliceerd in:
AntOBIS: Antarctic Ocean Biodiversity Information System, meer
(Gedeeltelijk) opgenomen in:
RAS: Register of Antarctic Species, meer
Dataset status: Afgelopen
Data type: Meta database
Data oorsprong: Onderzoek: veldonderzoek
Metadatarecord aangemaakt: 2019-04-08
Informatie laatst gewijzigd: 2019-04-10
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