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Characterization of the tilapia ßFSH gene and the analysis of its splice variants
Rosenfeld, H.; Meiri, I.; Melamed, P.; Gur, G.; Yaron, Z.; Elizur, A. (2000). Characterization of the tilapia ßFSH gene and the analysis of its splice variants, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 484
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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Keyword
    Marine/Coastal
Authors  Top 
  • Rosenfeld, H.
  • Meiri, I.
  • Melamed, P.
  • Gur, G.
  • Yaron, Z.
  • Elizur, A.
Abstract
    The full length sequence of the beta FSH gene of tilapia (Oreochromis mossambicus) was determined. Its transcriptional unit spans 2.7 kb and contains two introns: the first (1256bp) is within the 5' untranslated region while the second (957 bp) is in the conserved position at the 38th amino acid of the mature peptide. RT-PCR studies revealed that beta FSH gene expression involves two mRNA transcripts produced by alternate splicing of the first intron, without altering the peptide sequence. Similar splicing patterns were found also in other tilapia species and hybrids indicating that this phenomenon is not species specific. A competitive RT-PCR (CRT-PCR) was developed to evaluate the ratio of the variants. Our current data indicate that although the total amount of beta FSH transcript was increased in response to GnRH (0.1 nM), the ratio of the two splice variants remained constant.
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