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Production of recombinant striped bass (Morone saxatilis) gonadotropin in Chinese hamster ovarian (CHO) cell expression system
Blaise, O.; Szkudlindski, M.; Hassin, S.; Stubblefield, J.; Weintraub, B.; Zohar, Y. (2000). Production of recombinant striped bass (Morone saxatilis) gonadotropin in Chinese hamster ovarian (CHO) cell expression system, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 482
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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Keyword
    Marine/Coastal
Authors  Top 
  • Blaise, O.
  • Szkudlindski, M.
  • Hassin, S.
  • Stubblefield, J.
  • Weintraub, B.
  • Zohar, Y.
Abstract
    In this paper, we present the production of recombinant striped bass (Morone saxatilis) LH and FSH as well as LH beta and FSH beta subunits. These proteins were produced in CHO-K1 cells using the pcDNA3.1/zeo+ vector. We confirmed the presence of the LH by Western Blot and ELISA and show that this protein is glycosylated. We detected the presence of the FSH in Western Blot by cross reactivity with the anti LH beta . Both recombinant hormones are biologically actives.
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