Differential expression and structure of the goldfish FSH-B (GTH-Iß) and LHß (GTH-IIß) genes
Sohn, Y.C.; Kobayashi, A.; Aida, K. (2000). Differential expression and structure of the goldfish FSH-B (GTH-Iß) and LHß (GTH-IIß) genes, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 469-471
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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| Authors | | Top |
- Sohn, Y.C.
- Kobayashi, A.
- Aida, K.
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| Abstract |
To understand the steroidal regulation of FSH (GTH-I) and LH (GTH-II) at the molecular level in fish, we examined gene expression of FSH beta and LH beta subunits, and characterized the FSH beta and LH beta genes including their 5'-flanking regions in goldfish. Testosterone or estradiol-17 beta , when given in vivo, exerted an inhibitory effect on FSH beta gene expression but a stimulatory effect on LH beta in immature goldfish. In the 5'-flanking regions of the two genes (approximately 1.4 kb), several putative cis-acting elements were found, including the gonadotrope-specific element, gonadotropin-releasing hormone responsive element, activating protein-1 responsive element, and half steroid hormone responsive elements. The location of the putative regulatory elements was found to differ between the 5'-flanking regions of the FSH beta and LH beta genes, suggesting that their gene expression are differentially regulated by sex steroids, via their upstream regulatory elements. Based on the location of their first intron, goldfish FSH beta and LH beta genes were included in mammalian FSH beta /TSH beta group and LH beta group, respectively. The gene structures of goldfish gonadotropin beta subunits may support a previous suggestion that the nomenclatures of FSH and LH should be adopted instead of GTH-I and GTH-II. |
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