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Sperm cryopreservation of African catfish, Clarias gariepinus
Viveiros, A.T.M.; Komen, J. (2000). Sperm cryopreservation of African catfish, Clarias gariepinus, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 416
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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Keyword
    Marine/Coastal
Authors  Top 
  • Viveiros, A.T.M.
  • Komen, J.
Abstract
    Several protocols were tested to freeze and maximise the use of African Catfish sperm. Best results were obtained by sperm frozen with Methanol 10%, at 5 degree C/min until -40 degree C, held for 5min, then stored in liquid nitrogen (NL sub(2)) and post-thawed diluted to 1:200. This method was also tested under on-farm fertilisation conditions and produced hatching rates similar (P>0.05) to fresh sperm.
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