Long-term effects of nonylphenol on vitellogenin and zona radiata protein expression in juvenile rainbow trout
Fent, K.; Ackermann, G.; Schwaiger, J. (2000). Long-term effects of nonylphenol on vitellogenin and zona radiata protein expression in juvenile rainbow trout, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 356-358
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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| Authors | | Top |
- Fent, K.
- Ackermann, G.
- Schwaiger, J.
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| Abstract |
Environmental effects of environmental disruptors on fish have been shown. Here, we assess the estrogenic effects of 1 and 10 µg/l nonylphenol (NP) on vitellogenin (VG) and zona radiata protein (Zrp) expression in rainbow trout (Oncorhynchus mykiss) after a 12 months exposure. Trout eggs, larvae and juvenile stages were exposed in a flow-through system. VG mRNA, VG and ZrP protein were quantified by competitive RT-PCR and western blots, respectively. An increased VG protein concentration was detected at 1 µg/l NP, VG mRNA and Zrp content were significantly increased at 10 µg/l NP. This study shows that NP concentrations found in sewage in Switzerland and England induce estrogenic effects in trout, which indicates the LOEC to be set at 1 µg/l NP. |
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