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A simplified in vitro culture technique for the analysis of vitellogenin gene expression in liver slices of Japanese eel, Anguilla japonica
Adachi, S.; Abe, Y.; Koeda, Y.; Okumura, H.; Nakamua, I.; Yamauchi, K. (2000). A simplified in vitro culture technique for the analysis of vitellogenin gene expression in liver slices of Japanese eel, Anguilla japonica, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 300
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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Keyword
    Marine/Coastal
Authors  Top 
  • Adachi, S.
  • Abe, Y.
  • Koeda, Y.
  • Okumura, H.
  • Nakamua, I.
  • Yamauchi, K.
Abstract
    A simplified in vitro culture system, using liver slices, was attempted to analyze the endocrine control of egg yolk precursor proteins (vitellogenin; VTG) gene expression in Japanese eel, Anguilla japonica. A significant increase in VTG mRNA levels was detected by RT-PCR only in tissues incubated with estradiol-17 beta (E2) in the presence of bovine serum albumin (BSA), suggesting that BSA plays a role in this system.
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