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Aromatase gene expression in temperature-induced gonadal sex differentiation of tilapia Oreochromis niloticus
D'Cotta, H.; Guiguen, Y.; Govoroun, M.; McMeel, O.M.; Baroiller, J.F. (2000). Aromatase gene expression in temperature-induced gonadal sex differentiation of tilapia Oreochromis niloticus, in: Norberg, B. et al. (Ed.) Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. pp. 244-246
In: Norberg, B. et al. (2000). Proceedings of the 6th International Symposium on the Reproductive Physiology of Fish, Bergen, Norway, July 4-9, 1999. International Symposium on the Reproductive Physiology of Fish, 6. Department of Fisheries and Marine Biology, University of Bergen: Bergen. ISBN 82-7461-048-2. 499 pp., more
In: International Symposium on the Reproductive Physiology of Fish. Museo Nacional de Ciencias Naturales. , more
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Keyword
    Marine/Coastal
Authors  Top 
  • D'Cotta, H.
  • Guiguen, Y.
  • Govoroun, M.
  • McMeel, O.M.
  • Baroiller, J.F.
Abstract
    During gonadal sex differentiation of tilapia Oreochromis niloticus, progenies can be functionally masculinized by high rearing temperatures of 35 degree C when applied throughout the thermosensitive period. In teleost fish, aromatase seems to be a key enzyme for ovarian differentiation. Aromatase gene expression was assessed to determine its implication in the gonadal sex differentiation induced by temperature in tilapia. Genetically all-female populations sired from XX males and all-male populations sired from YY males (negative control) were reared at both 27 degree C (control) and 35 degree C (masculinizing treatment). Dissection of gonads was performed in both groups before histological gonadal sex differentiation. A tilapia aromatase cDNA of 1300 bp was obtained by screening an ovary cDNA library. Gene expression was analysed by virtual Northern blots using this probe and by semi-quantitative RT-PCR. Higher levels of aromatase gene expression were revealed in all-female populations when compared to males. Progenies in which temperature caused total sex inversion of gonads, showed a decrease in aromatase expression in treated groups
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